Hibiscus rosa-sinensis is an important traditional plant with immense therapeutic value. To evaluate the scientific basis for the use of this plant, the phytochemical screening and its antibacterial activity was experimentally carried out. The aqueous crude extract of Hibiscus rosa-sinensis leaves were screened for its phytoconstituents and this extract revealed for the presence of saponins, phenols, alkaloids, proteins/amino acids, tannins, flavonoids, carbohydrates/reducing sugars and resins. These phytochemicals exhibits certain antibacterial activity which was further analyzed. The antibacterial activity of different concentrations of aqueous H.rosa-sinensis’ leaves extract were investigated in-vitro against Aeromonas hydrophila by the agar well diffusion method. The results indicated that all concentrations showed an antibacterial activity against the tested bacteria. The highest activity was observed with an inhibition zone of 24 mm at 50% concentration.